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Question:

A protoplast is a cell without cell wall, without plasma membrane, undergoing division, or without nucleus?

Without plasma membrane

Without nucleus

Without cell wall

Undergoing division

Solution:

Protoplasts are cells with the plasma membrane, cytoplasm and nucleus, which have had their cell wall removed by the action of enzymes. Cellulase enzymes digest the cellulose in plant cell walls while pectinase enzymes break down the pectin holding cells together. Once the cell wall has been removed the resulting protoplast is spherical in shape.