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Question:

How does a restriction nuclease function?

Solution:

Restriction endonuclease, is the most widely used enzyme for the process of gene modification. It recognizes a specific sequence of nucleotides which is known as recognition site and produces a double strand nick in the respective DNA. The action of restriction endonuclease can be on palindromic sequences. For example, EcoRI cuts the DNA at the following palindromic sequence: 5' GAATTC 3' 3' CTTAA G 5'