devarshi-dt-logo

Question:

How is the amplification of a gene sample of interest carried out using Polymerase Chain Reaction (PCR)?

Solution:

Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps:
Denaturation: The double-stranded DNA is heated up to 94oC which causes the hydrogen bonds to break and the two strands get separated.
Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand at 540C.
Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand at 720C.