Plasmolyse the cell and filter out the mixture and take the debris
Select cells which have a larger number of mitochondria
Subject the cell to cell fractionation (centrifuge) and obtain mitochondria
Grind the cells and filter out the mixture and take the debris
Although biochemical analysis requires disruption of the anatomy of the cell, but one needs to separate the various cell components while preserving their individual functions.Hence, plasmolysis and grinding the cell can not fulfill the purpose as it will disrupt the structure of organelles.Gentle centrifugation techniques can separate the cell organelles on the basis of their sedimentation coefficient without damaging their structure and can be used to obtain mitochondria for biochemical analysis.So, the correct answer is option D.