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Question:

In which of the following reactions of glycolysis, a molecule of water is removed from the substrate?

2-phosphoglycerate→Phosphoenol pyruvate

Glucose→Glucose-6-phosphate

Fructose-6-phosphate→Fructose-1,6 bisphosphate

Phosphoenol pyruvate→Pyruvate

Solution:

Options A and B represent the addition of phosphate group to glucose and fructose 6 phosphates respectively. Option D represents dephosphorylation of phosphoenolpyruvate into pyruvate. Dehydration of 2-phosphoglycerate to phosphoenolpyruvate includes reversible removal of a molecule of water from 2-phosphoglycerate. So, the correct answer is option C.