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Question:

Nitrogenase requires..........for its action. Mn2+, Super oxygen radicals, Light, High input of energy

Light

Super oxygen radicals

Mn2+

High input of energy

Solution:

The enzyme nitrogenase is found in certain bacteria and blue-green algae, which can reduce N2 to NH3 (nitrogen fixation).
N2 + 8H+ + 8e− + 16ATP Nitrogenase → 2NH3 + H2 + 16ADP
The equilibrium formation of ammonia from molecular hydrogen and nitrogen has an overall negative enthalpy of reaction, the activation energy is very high, so, the reaction requires high energy input.