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Question:

Rearrange the following in the current sequences to accomplish an important biotechnological reaction.(a) In vitro synthesis of copies of DNA of interest(b) Chemically synthesized oligonucleotides(c) Enzyme DNA-polymerase(d) Complementary region of DNA(e) Genomic DNA template(f) Nucleotides provided(g) Primers(h) Thermostable DNA-polymerase (from Thermus aquaticus)(i) Denaturation of ds-DNA

Solution:

The correct sequence of the steps involved in biotechnological reaction is:
Genomic DNA template
Denaturation of ds-DNA
Primers
Nucleotides provided
Thermostable DNA polymerase (from Thermus aquaticus)
Enzyme DNA polymerase
Chemically synthesised oligonucleotides
Complementary region of DNA
In vitro synthesis of copies of DNA of interest