Recombinant DNA technology involves several steps. The initial step is the isolation of DNA. Which enzymes are used in the process for the breakdown of fungal cells, plant cells, and bacterial cells respectively?

Processes of recombinant DNA technology involve isolation of DNA of a desired DNA fragment. In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules. The DNA is enclosed within the membranes; we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides, and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus). The RNA can be removed by treatment with ribonuclease, whereas proteins can be removed by treatment with protease.