Eduprobe
Question:
The correct order of steps in Polymerase Chain Reaction (PCR) is Denaturation, Annealing, Extension
Denaturation, Extension, Annealing
Extension, Denaturation, Annealing
Denaturation, Annealing, Extension
Annealing, Extension, Denaturation
Solution:
The correct option is C Denaturation, Annealing, Extension
Polymerase chain reaction or PCR consists of the following three steps:
Denaturation- The two DNA strands of template DNA separate from each other when heated to 92°C.
Annealing- The primers anneal to the 3’ end of single strands of DNA.
Extension- The primers are extended by DNA polymerase by the addition of nucleotides to form complete strands of DNA. Hence the sequence of steps is denaturation, annealing, extension.
So, the correct answer is option C.