The outermost limiting layer of Mycoplasma is made up of Cell wall. Cell membrane. Mucilaginous sheath. Slime layer.

Mycoplasma is the smallest and simplest free-living Gram-negative prokaryotes or monerans. A cell wall is absent. Plasma membrane forms the outer boundary of the cell. A substantial amount of polysaccharides having acetyl glucosamine is associated with the cell membrane.